268738

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 5749830..5751559 Replicon chromosome
Accession NZ_CP116478
Organism Pseudomonas syringae pv. actinidiae strain PSA.AH.01

Toxin (Protein)


Gene name pmenT Uniprot ID A0A2V0QPP4
Locus tag PHA47_RS25645 Protein ID WP_003379114.1
Coordinates 5749830..5750474 (+) Length 215 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A2V0QNZ0
Locus tag PHA47_RS25650 Protein ID WP_003379115.1
Coordinates 5750471..5751559 (+) Length 363 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PHA47_RS25620 (PHA47_25620) 5744889..5745698 + 810 WP_017683789.1 zinc-dependent peptidase -
PHA47_RS25625 (PHA47_25625) 5745809..5746336 + 528 WP_003379107.1 inorganic diphosphatase -
PHA47_RS25630 (PHA47_25630) 5746682..5747413 + 732 WP_003379109.1 XRE family transcriptional regulator -
PHA47_RS25635 (PHA47_25635) 5747713..5748834 - 1122 WP_003379112.1 endonuclease NucS -
PHA47_RS25640 (PHA47_25640) 5749252..5749833 + 582 WP_017683790.1 DarT ssDNA thymidine ADP-ribosyltransferase family protein -
PHA47_RS25645 (PHA47_25645) 5749830..5750474 + 645 WP_003379114.1 DUF4433 domain-containing protein Toxin
PHA47_RS25650 (PHA47_25650) 5750471..5751559 + 1089 WP_003379115.1 macro domain-containing protein Antitoxin
PHA47_RS25655 (PHA47_25655) 5751872..5752018 - 147 WP_017699797.1 hypothetical protein -
PHA47_RS25660 (PHA47_25660) 5752099..5752803 - 705 WP_017683791.1 ABC transporter ATP-binding protein -
PHA47_RS25665 (PHA47_25665) 5752800..5753576 - 777 WP_017699458.1 ABC transporter permease -
PHA47_RS25670 (PHA47_25670) 5753743..5754809 - 1067 Protein_5066 IS630-like element ISPsy32 family transposase -
PHA47_RS25675 (PHA47_25675) 5755080..5755922 + 843 WP_003379119.1 glutathione-dependent disulfide-bond oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 215 a.a.        Molecular weight: 24813.38 Da        Isoelectric Point: 6.4920

>T268738 WP_003379114.1 NZ_CP116478:5749830-5750474 [Pseudomonas syringae pv. actinidiae]
MIYSSLNPEKALIWRIVHRDNLPWILDNGLHCASSYMQAPQYVNIGNADLIDKRRNRSVPIAPAGVLSDYVPFYFTPFSV
MMKNIHSGWSVQQRSNDEIVILVSSLPRIEQLGLAFVFTNAHAYPDWTDYYSDLVNLDQIDWPIIQKRDFKRDPDDPRKM
ERYQAEALIHHHLPITGLLGIMCYDDAMKERIEQDVAARGLTLPVHARPGWYFQ

Download         Length: 645 bp

Antitoxin


Download         Length: 363 a.a.        Molecular weight: 40646.66 Da        Isoelectric Point: 6.1829

>AT268738 WP_003379115.1 NZ_CP116478:5750471-5751559 [Pseudomonas syringae pv. actinidiae]
MIRFTQGNLLEADTEALVNTVNTVGVMGKGIALMFKERFTENYRLYSAACKAGEVETGKVHVTAVNELDGPRWIVNFPTK
RHWRSPSQMAWITEGLRDLRRFLIDNHVKSVAVPPLGAGNGGLKWVEVREQIVDVLSDLDVDVLVFEPTDQYLNVAKRSG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERSIEQLPSAKNPLDLKFVAHKYGPYANRLEHLLDNLDGSYLH
CDKRISDAGIDDVIWFDEGRKAFLQTYLKTEAKEYSQALERTAELIDGFESPFGMELLATVDWLLCKSGVSPTVPTVREA
LKHWDGGAGAAARKSRLFDDKAIDIALKRLTTSSLSPEMPPS

Download         Length: 1089 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0QPP4

Antitoxin

Source ID Structure
AlphaFold DB A0A2V0QNZ0

References