Detailed information of TA system TA026870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3097264..3097834
• Replicon: chromosome
• Accession: NC_017522
• Organism: Mycobacterium tuberculosis CCDC5180

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: CCDC5180_RS14780
• Protein ID: WP_003414166.1
• Coordinates: 3097264..3097620 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: CCDC5180_RS14785
• Protein ID: WP_003901465.1
• Coordinates: 3097604..3097834 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCDC5180_RS14760	3092716..3094404	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
CCDC5180_RS14765	3094408..3094734	-	327	WP_003414157.1	hypothetical protein	-
CCDC5180_RS14770	3094907..3095494	+	588	WP_003914429.1	DUF3558 family protein	-
CCDC5180_RS14775	3095513..3097162	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
CCDC5180_RS14780	3097264..3097620	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
CCDC5180_RS14785	3097604..3097834	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
CCDC5180_RS14790	3097877..3098920	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
CCDC5180_RS22370	3099111..3099386	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
CCDC5180_RS22530	3099562..3099816	-	255	WP_003917684.1	hypothetical protein	-
CCDC5180_RS14805	3099964..3100368	+	405	WP_003414181.1	hypothetical protein	-
CCDC5180_RS14810	3100365..3100556	+	192	WP_003414184.1	hypothetical protein	-
CCDC5180_RS14815	3100620..3101909	+	1290	Protein_2892	transposase family protein	-
CCDC5180_RS14820	3102143..3102400	+	258	WP_003899489.1	hypothetical protein	-
CCDC5180_RS14825	3102505..3102816	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T26870 WP_003414166.1 NC_017522:c3097620-3097264 [Mycobacterium tuberculosis CCDC5180]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T26870 NC_017522:c3097620-3097264 [Mycobacterium tuberculosis CCDC5180]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT26870 WP_003901465.1 NC_017522:c3097834-3097604 [Mycobacterium tuberculosis CCDC5180]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT26870 NC_017522:c3097834-3097604 [Mycobacterium tuberculosis CCDC5180]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3