Detailed information of TA system TA026869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3057681..3058368
• Replicon: chromosome
• Accession: NC_017522
• Organism: Mycobacterium tuberculosis CCDC5180

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: CCDC5180_RS14570
• Protein ID: WP_003414064.1
• Coordinates: 3057973..3058368 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: CCDC5180_RS14565
• Protein ID: WP_003414061.1
• Coordinates: 3057681..3057947 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCDC5180_RS14540	3053320..3054222	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CCDC5180_RS14545	3054291..3055043	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
CCDC5180_RS14550	3055287..3055562	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
CCDC5180_RS14555	3055559..3057181	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
CCDC5180_RS14560	3057268..3057684	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
CCDC5180_RS14565	3057681..3057947	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CCDC5180_RS14570	3057973..3058368	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
CCDC5180_RS14575	3058365..3058634	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
CCDC5180_RS14580	3058644..3059738	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
CCDC5180_RS14585	3059735..3060154	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
CCDC5180_RS14590	3060228..3060707	-	480	WP_003414073.1	dihydrofolate reductase	-
CCDC5180_RS14595	3060778..3061578	-	801	WP_003911953.1	thymidylate synthase	-
CCDC5180_RS14600	3061734..3062471	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T26869 WP_003414064.1 NC_017522:c3058368-3057973 [Mycobacterium tuberculosis CCDC5180]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T26869 NC_017522:c3058368-3057973 [Mycobacterium tuberculosis CCDC5180]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT26869 WP_003414061.1 NC_017522:c3057947-3057681 [Mycobacterium tuberculosis CCDC5180]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT26869 NC_017522:c3057947-3057681 [Mycobacterium tuberculosis CCDC5180]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64