Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 87301..87826 Replicon plasmid pHH97
Accession NZ_CP116410
Organism Escherichia coli strain HH97

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag PL329_RS24465 Protein ID WP_271711335.1
Coordinates 87301..87606 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag PL329_RS24470 Protein ID WP_000813634.1
Coordinates 87608..87826 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PL329_RS24435 (82567) 82567..83837 + 1271 Protein_103 translesion error-prone DNA polymerase V subunit UmuC -
PL329_RS24440 (83842) 83842..84234 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
PL329_RS24445 (84239) 84239..85210 - 972 WP_001103694.1 plasmid segregation protein ParM -
PL329_RS24450 (85439) 85439..86083 + 645 WP_271711334.1 AAA family ATPase -
PL329_RS24455 (86077) 86077..86352 + 276 WP_000239529.1 hypothetical protein -
PL329_RS24460 (86491) 86491..87300 - 810 WP_000016979.1 site-specific integrase -
PL329_RS24465 (87301) 87301..87606 - 306 WP_271711335.1 type II toxin-antitoxin system toxin CcdB Toxin
PL329_RS24470 (87608) 87608..87826 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
PL329_RS24475 (90147) 90147..90788 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..114684 114684


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11702.53 Da        Isoelectric Point: 6.4674

>T268679 WP_271711335.1 NZ_CP116410:c87606-87301 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVPVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT268679 WP_000813634.1 NZ_CP116410:c87826-87608 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References