Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4734557..4735054 Replicon chromosome
Accession NZ_CP116409
Organism Escherichia coli strain HH97

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PL329_RS23165 Protein ID WP_001173343.1
Coordinates 4734557..4734730 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag PL329_RS23170 Protein ID WP_000398619.1
Coordinates 4734758..4735054 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PL329_RS23155 (4731227) 4731227..4732684 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
PL329_RS23160 (4732921) 4732921..4734438 + 1518 WP_001295074.1 lysine--tRNA ligase -
PL329_RS23165 (4734557) 4734557..4734730 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PL329_RS23170 (4734758) 4734758..4735054 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PL329_RS23175 (4735281) 4735281..4735553 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
PL329_RS23180 (4735565) 4735565..4735795 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
PL329_RS23185 (4735976) 4735976..4737607 + 1632 WP_001216477.1 sensor histidine kinase -
PL329_RS23190 (4737604) 4737604..4738323 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4710658..4735054 24396


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T268678 WP_001173343.1 NZ_CP116409:c4734730-4734557 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT268678 WP_000398619.1 NZ_CP116409:c4735054-4734758 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References