Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2857927..2858564 Replicon chromosome
Accession NC_017522
Organism Mycobacterium tuberculosis CCDC5180

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag CCDC5180_RS13495 Protein ID WP_003413180.1
Coordinates 2857927..2858322 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag CCDC5180_RS13500 Protein ID WP_003413183.1
Coordinates 2858319..2858564 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCDC5180_RS13460 2853331..2854542 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
CCDC5180_RS13465 2854669..2855328 + 660 WP_003902296.1 hypothetical protein -
CCDC5180_RS13470 2855325..2855987 + 663 WP_003905878.1 hypothetical protein -
CCDC5180_RS22355 2855984..2856261 + 278 Protein_2623 type II toxin-antitoxin system VapB family antitoxin -
CCDC5180_RS13475 2856354..2856767 + 414 WP_003413164.1 PIN domain nuclease -
CCDC5180_RS13480 2856806..2857063 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
CCDC5180_RS13485 2857060..2857437 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
CCDC5180_RS13490 2857453..2857827 - 375 WP_003413177.1 hypothetical protein -
CCDC5180_RS13495 2857927..2858322 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
CCDC5180_RS13500 2858319..2858564 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
CCDC5180_RS13505 2858975..2859394 - 420 WP_003413190.1 A24 family peptidase -
CCDC5180_RS13510 2859406..2860215 - 810 WP_003413193.1 shikimate dehydrogenase -
CCDC5180_RS13515 2860212..2861465 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
CCDC5180_RS13520 2861458..2861970 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T26865 WP_003413180.1 NC_017522:c2858322-2857927 [Mycobacterium tuberculosis CCDC5180]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T26865 NC_017522:c2858322-2857927 [Mycobacterium tuberculosis CCDC5180]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT26865 WP_003413183.1 NC_017522:c2858564-2858319 [Mycobacterium tuberculosis CCDC5180]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT26865 NC_017522:c2858564-2858319 [Mycobacterium tuberculosis CCDC5180]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References