Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/FR47-DUF1778
Location 3302..4099 Replicon plasmid unnamed3
Accession NZ_CP116350
Organism Enterobacter ludwigii strain CM-TZ4

Toxin (Protein)


Gene name KacT Uniprot ID F5S3R9
Locus tag PHA72_RS27710 Protein ID WP_006812498.1
Coordinates 3575..4099 (+) Length 175 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A5D4NAG3
Locus tag PHA72_RS27705 Protein ID WP_016582626.1
Coordinates 3302..3571 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PHA72_RS27685 (PHA72_27680) 1..1056 + 1056 WP_059306389.1 hypothetical protein -
PHA72_RS27690 (PHA72_27685) 1122..1847 - 726 WP_059306388.1 DUF2971 domain-containing protein -
PHA72_RS27695 (PHA72_27690) 1900..2253 - 354 WP_161574040.1 hypothetical protein -
PHA72_RS27700 (PHA72_27695) 2259..2924 - 666 WP_059306387.1 AAA family ATPase -
PHA72_RS27705 (PHA72_27700) 3302..3571 + 270 WP_016582626.1 DUF1778 domain-containing protein Antitoxin
PHA72_RS27710 (PHA72_27705) 3575..4099 + 525 WP_006812498.1 GNAT family N-acetyltransferase Toxin
PHA72_RS27715 (PHA72_27710) 4284..4535 - 252 WP_000147960.1 hypothetical protein -
PHA72_RS27720 (PHA72_27715) 4537..5229 - 693 WP_059306812.1 hypothetical protein -
PHA72_RS27725 (PHA72_27720) 5243..5566 - 324 WP_000064173.1 hypothetical protein -
PHA72_RS27730 (PHA72_27725) 5641..6363 - 723 WP_081051074.1 receptor-recognizing protein -
PHA72_RS27735 (PHA72_27730) 6401..6961 - 561 Protein_10 hypothetical protein -
PHA72_RS27740 (PHA72_27735) 7036..7419 - 384 WP_000469441.1 minor capsid protein -
PHA72_RS27745 (PHA72_27740) 7421..7894 - 474 WP_000523626.1 hypothetical protein -
PHA72_RS27750 (PHA72_27745) 7885..8229 - 345 WP_001027662.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..61089 61089


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 175 a.a.        Molecular weight: 19356.13 Da        Isoelectric Point: 8.9844

>T268616 WP_006812498.1 NZ_CP116350:3575-4099 [Enterobacter ludwigii]
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK

Download         Length: 525 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10113.57 Da        Isoelectric Point: 5.8580

>AT268616 WP_016582626.1 NZ_CP116350:3302-3571 [Enterobacter ludwigii]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0QWY8

Antitoxin

Source ID Structure
AlphaFold DB A0A5D4NAG3

References