Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 5097150..5097792 Replicon chromosome
Accession NZ_CP116325
Organism Bacillus thuringiensis strain BLB1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag PJW01_RS26635 Protein ID WP_000635965.1
Coordinates 5097442..5097792 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag PJW01_RS26630 Protein ID WP_000004570.1
Coordinates 5097150..5097437 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PJW01_RS26605 (PJW01_26605) 5092467..5093429 + 963 WP_000961167.1 UV DNA damage repair endonuclease UvsE -
PJW01_RS26610 (PJW01_26610) 5093422..5093994 - 573 WP_000908522.1 rhomboid family intramembrane serine protease -
PJW01_RS26615 (PJW01_26615) 5094088..5094447 + 360 WP_000583417.1 holo-ACP synthase -
PJW01_RS26620 (PJW01_26620) 5094604..5095554 + 951 WP_002100659.1 outer membrane lipoprotein carrier protein LolA -
PJW01_RS26625 (PJW01_26625) 5095672..5096841 + 1170 WP_000390616.1 alanine racemase -
PJW01_RS26630 (PJW01_26630) 5097150..5097437 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
PJW01_RS26635 (PJW01_26635) 5097442..5097792 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PJW01_RS26640 (PJW01_26640) 5097860..5100028 + 2169 WP_000426236.1 Tex family protein -
PJW01_RS26645 (PJW01_26645) 5100087..5100203 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
PJW01_RS26650 (PJW01_26650) 5100399..5100857 + 459 WP_000344239.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T268594 WP_000635965.1 NZ_CP116325:5097442-5097792 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT268594 WP_000004570.1 NZ_CP116325:5097150-5097437 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References