Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 3484571..3485549 Replicon chromosome
Accession NZ_CP116313
Organism Bacillus thuringiensis strain Lip

Toxin (Protein)


Gene name spoIISA Uniprot ID W8Y388
Locus tag PJW02_RS18000 Protein ID WP_000624977.1
Coordinates 3484812..3485549 (-) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID W8YZL5
Locus tag PJW02_RS17995 Protein ID WP_000237818.1
Coordinates 3484571..3484699 (-) Length 43 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PJW02_RS17965 (PJW02_17965) 3480059..3480310 + 252 WP_000827560.1 LuxR C-terminal-related transcriptional regulator -
PJW02_RS17970 (PJW02_17970) 3480550..3481152 - 603 WP_000517260.1 hypothetical protein -
PJW02_RS17975 (PJW02_17975) 3481178..3481987 - 810 WP_000678509.1 papain-like cysteine protease family protein -
PJW02_RS17980 (PJW02_17980) 3482233..3483702 - 1470 WP_000287515.1 beta-Ala-His dipeptidase -
PJW02_RS17985 (PJW02_17985) 3483914..3484303 + 390 WP_000713866.1 YxeA family protein -
PJW02_RS17990 (PJW02_17990) 3484322..3484498 - 177 WP_000852629.1 stage II sporulation protein SB -
PJW02_RS17995 (PJW02_17995) 3484571..3484699 - 129 WP_000237818.1 hypothetical protein Antitoxin
PJW02_RS18000 (PJW02_18000) 3484812..3485549 - 738 WP_000624977.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
PJW02_RS18005 (PJW02_18005) 3485787..3486524 - 738 WP_000594152.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
PJW02_RS18010 (PJW02_18010) 3486691..3487173 - 483 WP_000191888.1 MarR family winged helix-turn-helix transcriptional regulator -
PJW02_RS18015 (PJW02_18015) 3487281..3488966 + 1686 WP_003284281.1 alpha-keto acid decarboxylase family protein -
PJW02_RS18020 (PJW02_18020) 3489124..3489906 - 783 WP_000381800.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28307.03 Da        Isoelectric Point: 8.2998

>T268588 WP_000624977.1 NZ_CP116313:c3485549-3484812 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

Antitoxin


Download         Length: 43 a.a.        Molecular weight: 5011.40 Da        Isoelectric Point: 3.9150

>AT268588 WP_000237818.1 NZ_CP116313:c3484699-3484571 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Download         Length: 129 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W8Y388

Antitoxin

Source ID Structure
AlphaFold DB W8YZL5

References