268587

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 179523..180165 Replicon chromosome
Accession NZ_CP116313
Organism Bacillus thuringiensis strain Lip

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag PJW02_RS00930 Protein ID WP_000635965.1
Coordinates 179523..179873 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag PJW02_RS00935 Protein ID WP_000004570.1
Coordinates 179878..180165 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PJW02_RS00915 (PJW02_00915) 176458..176916 - 459 WP_000344239.1 SprT family protein -
PJW02_RS00920 (PJW02_00920) 177112..177228 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
PJW02_RS00925 (PJW02_00925) 177287..179455 - 2169 WP_000426236.1 Tex family protein -
PJW02_RS00930 (PJW02_00930) 179523..179873 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PJW02_RS00935 (PJW02_00935) 179878..180165 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
PJW02_RS00940 (PJW02_00940) 180474..181643 - 1170 WP_000390616.1 alanine racemase -
PJW02_RS00945 (PJW02_00945) 181761..182711 - 951 WP_002100659.1 outer membrane lipoprotein carrier protein LolA -
PJW02_RS00950 (PJW02_00950) 182868..183227 - 360 WP_000583417.1 holo-ACP synthase -
PJW02_RS00955 (PJW02_00955) 183321..183893 + 573 WP_000908522.1 rhomboid family intramembrane serine protease -
PJW02_RS00960 (PJW02_00960) 183886..184848 - 963 WP_000961167.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T268587 WP_000635965.1 NZ_CP116313:c179873-179523 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT268587 WP_000004570.1 NZ_CP116313:c180165-179878 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References