Detailed information of TA system TA268580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 1201124..1201735
• Replicon: chromosome
• Accession: NZ_CP116304
• Organism: Pseudomonas sp. Q1-7

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: PJW05_RS05550
• Protein ID: WP_271410733.1
• Coordinates: 1201124..1201414 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: PJW05_RS05555
• Protein ID: WP_271410734.1
• Coordinates: 1201424..1201735 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PJW05_RS05530	1198059..1198535	+	477	WP_271410730.1	pilin	-
PJW05_RS05535	1198606..1200270	+	1665	WP_271410731.1	O-antigen ligase family protein	-
PJW05_RS05545	1200447..1200674	-	228	WP_271410732.1	hypothetical protein	-
PJW05_RS05550	1201124..1201414	+	291	WP_271410733.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PJW05_RS05555	1201424..1201735	+	312	WP_271410734.1	putative addiction module antidote protein	Antitoxin
PJW05_RS05560	1201949..1202797	-	849	WP_271410735.1	carboxylating nicotinate-nucleotide diphosphorylase	-
PJW05_RS05565	1203113..1205383	+	2271	WP_271410736.1	DUF1631 domain-containing protein	-
PJW05_RS05570	1205585..1206133	+	549	WP_271410737.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-92)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10733.26 Da
• Isoelectric Point: 6.9786

>T268580 WP_271410733.1 NZ_CP116304:1201124-1201414 [Pseudomonas sp. Q1-7]
MKSILTTDTFDSWFAGLRDKRAVMRIQARIDRAEGGNFGDCEPVGEGVSEMRIHYGPGYRVYFIQRGLEIVILLAGGEKS
TQQNDIKAAIDIARQL

Nucleotide

• Length: 291 bp

>T268580 NZ_CP116304:1201124-1201414 [Pseudomonas sp. Q1-7]
ATGAAGTCTATTCTCACCACGGACACTTTCGATTCATGGTTCGCCGGCCTCCGGGACAAGCGGGCCGTCATGCGCATCCA
GGCGCGCATCGATCGAGCGGAGGGCGGCAACTTCGGCGACTGCGAGCCGGTCGGCGAGGGTGTTTCGGAGATGCGTATTC
ACTATGGGCCAGGTTATCGGGTGTACTTCATCCAACGGGGCCTGGAGATCGTGATCCTGCTGGCAGGCGGCGAAAAGTCT
ACCCAGCAAAACGATATCAAGGCCGCGATCGACATCGCGCGACAGCTTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11153.80 Da
• Isoelectric Point: 6.4633

>AT268580 WP_271410734.1 NZ_CP116304:1201424-1201735 [Pseudomonas sp. Q1-7]
MTTVKLRKWDSAEYLKTDEDMALYLEACIEEAGDDAAFIVRALGTIARARGMTQLARDTGLGRESLYKALSGESNPSFAT
ILKVTRALGIKLHAEVIPTHAAG

Nucleotide

• Length: 312 bp

>AT268580 NZ_CP116304:1201424-1201735 [Pseudomonas sp. Q1-7]
ATGACTACCGTCAAACTGCGCAAATGGGATAGCGCCGAATACCTCAAAACCGATGAGGACATGGCGCTCTACCTGGAAGC
CTGCATCGAGGAAGCCGGCGACGATGCGGCGTTCATTGTGAGGGCGCTGGGCACTATCGCCCGAGCCCGAGGTATGACGC
AGCTGGCGCGAGACACTGGTCTCGGGCGCGAAAGCCTGTACAAGGCTCTGTCAGGTGAAAGCAACCCGAGTTTCGCGACC
ATTCTCAAGGTGACGCGCGCGCTTGGCATCAAGCTTCACGCAGAAGTCATCCCCACCCACGCGGCGGGTTGA