Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4825..5561 Replicon plasmid pMCR10_120063
Accession NZ_CP116250
Organism Enterobacter roggenkampii strain 120063

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KK030_RS24560 Protein ID WP_003026803.1
Coordinates 5079..5561 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KK030_RS24555 Protein ID WP_003026799.1
Coordinates 4825..5091 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KK030_RS24530 (KK030_24480) 1..1011 + 1011 WP_000200070.1 RepB family plasmid replication initiator protein -
KK030_RS24535 (KK030_24485) 1709..2449 - 741 WP_001515717.1 tyrosine-type recombinase/integrase -
KK030_RS24540 (KK030_24490) 2813..3781 - 969 WP_271800736.1 IS5-like element IS903B family transposase -
KK030_RS24545 (KK030_24495) 3933..4136 + 204 WP_004213596.1 HHA domain-containing protein -
KK030_RS24550 (KK030_24500) 4150..4380 + 231 WP_023307222.1 hypothetical protein -
KK030_RS24555 (KK030_24505) 4825..5091 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KK030_RS24560 (KK030_24510) 5079..5561 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KK030_RS24565 (KK030_24515) 5773..7116 + 1344 WP_080339306.1 ISNCY family transposase -
KK030_RS24570 (KK030_24520) 7187..8005 - 819 WP_023307220.1 abortive infection family protein -
KK030_RS24575 (KK030_24525) 8014..8442 - 429 Protein_9 recombinase family protein -
KK030_RS24580 (KK030_24530) 8402..9433 - 1032 WP_001395480.1 IS630-like element ISEc33 family transposase -
KK030_RS24585 (KK030_24535) 9535..9741 - 207 Protein_11 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-10 - 1..195008 195008
- inside IScluster/Tn - - 2813..16574 13761


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T268563 WP_003026803.1 NZ_CP116250:5079-5561 [Enterobacter roggenkampii]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT268563 WP_003026799.1 NZ_CP116250:4825-5091 [Enterobacter roggenkampii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References