Detailed information of TA system TA026856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2244042..2244668
• Replicon: chromosome
• Accession: NC_017522
• Organism: Mycobacterium tuberculosis CCDC5180

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: CCDC5180_RS10565
• Protein ID: WP_003410075.1
• Coordinates: 2244270..2244668 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: CCDC5180_RS10560
• Protein ID: WP_003911750.1
• Coordinates: 2244042..2244269 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCDC5180_RS10550	2242081..2242425	-	345	WP_003410065.1	ferredoxin family protein	-
CCDC5180_RS10555	2242614..2243783	-	1170	WP_003899126.1	ATP-binding protein	-
CCDC5180_RS10560	2244042..2244269	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CCDC5180_RS10565	2244270..2244668	+	399	WP_003410075.1	PIN domain nuclease	Toxin
CCDC5180_RS10570	2244851..2245282	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
CCDC5180_RS10575	2245383..2245817	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
CCDC5180_RS21405	2246254..2246433	-	180	Protein_2067	hypothetical protein	-
CCDC5180_RS10580	2246494..2247141	+	648	WP_071854215.1	transposase	-
CCDC5180_RS10585	2247095..2247685	+	591	WP_003899131.1	IS110 family transposase	-
CCDC5180_RS10590	2247813..2249069	-	1257	WP_003902247.1	HNH endonuclease	-
CCDC5180_RS10595	2249423..2249623	+	201	Protein_2071	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T26856 WP_003410075.1 NC_017522:2244270-2244668 [Mycobacterium tuberculosis CCDC5180]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T26856 NC_017522:2244270-2244668 [Mycobacterium tuberculosis CCDC5180]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT26856 WP_003911750.1 NC_017522:2244042-2244269 [Mycobacterium tuberculosis CCDC5180]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT26856 NC_017522:2244042-2244269 [Mycobacterium tuberculosis CCDC5180]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8