Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 1887037..1887592 Replicon chromosome
Accession NZ_CP116249
Organism Enterobacter roggenkampii strain 120063

Toxin (Protein)


Gene name shpA Uniprot ID A0A839BSP9
Locus tag KK030_RS09520 Protein ID WP_071881499.1
Coordinates 1887037..1887303 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A839BUZ1
Locus tag KK030_RS09525 Protein ID WP_038416295.1
Coordinates 1887290..1887592 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KK030_RS09500 (KK030_09490) 1882511..1885795 + 3285 WP_063160719.1 type I restriction endonuclease subunit R -
KK030_RS09505 (KK030_09495) 1885792..1886547 + 756 WP_063135765.1 SprT family zinc-dependent metalloprotease -
KK030_RS09510 (KK030_09500) 1886634..1886708 + 75 Protein_1848 hypothetical protein -
KK030_RS09515 (KK030_09505) 1886681..1886992 - 312 Protein_1849 IS5/IS1182 family transposase -
KK030_RS09520 (KK030_09510) 1887037..1887303 + 267 WP_071881499.1 BrnT family toxin Toxin
KK030_RS09525 (KK030_09515) 1887290..1887592 + 303 WP_038416295.1 BrnA antitoxin family protein Antitoxin
KK030_RS09530 (KK030_09520) 1887713..1889056 - 1344 WP_038416294.1 APC family permease -
KK030_RS09535 (KK030_09525) 1889053..1889964 - 912 WP_038416293.1 acetamidase/formamidase family protein -
KK030_RS09540 (KK030_09530) 1890263..1891000 - 738 Protein_1854 IS5-like element IS5 family transposase -
KK030_RS09545 (KK030_09535) 1891038..1891724 + 687 Protein_1855 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 1886594..1899574 12980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(9-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(38-93)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10474.91 Da        Isoelectric Point: 10.0832

>T268553 WP_071881499.1 NZ_CP116249:1887037-1887303 [Enterobacter roggenkampii]
IRDLFAPSLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLSLVHRIIVIMVAHSVRFESGTEVIRIISARKADSK
ERNRYEHG

Download         Length: 267 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11637.21 Da        Isoelectric Point: 9.4429

>AT268553 WP_038416295.1 NZ_CP116249:1887290-1887592 [Enterobacter roggenkampii]
MNMVKHKRDNPDTLTTKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A839BSP9

Antitoxin

Source ID Structure
AlphaFold DB A0A839BUZ1

References