Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 82354..82955 Replicon plasmid unnamed4
Accession NZ_CP116245
Organism Escherichia coli strain 7.1994/NIST0056

Toxin (Protein)


Gene name doc Uniprot ID U9ZW91
Locus tag PH192_RS25160 Protein ID WP_001216033.1
Coordinates 82575..82955 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag PH192_RS25155 Protein ID WP_001190712.1
Coordinates 82354..82575 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PH192_RS25140 (PH192_25135) 78269..79459 - 1191 WP_097331719.1 anticodon nuclease -
PH192_RS25145 (PH192_25140) 79461..80618 - 1158 WP_097331718.1 restriction endonuclease subunit S -
PH192_RS25150 (PH192_25145) 80615..82171 - 1557 WP_097331735.1 type I restriction-modification system subunit M -
PH192_RS25155 (PH192_25150) 82354..82575 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
PH192_RS25160 (PH192_25155) 82575..82955 + 381 WP_001216033.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
PH192_RS25165 (PH192_25160) 82960..83139 + 180 WP_001339207.1 hypothetical protein -
PH192_RS25170 (PH192_25165) 83167..84210 + 1044 WP_063100003.1 DUF968 domain-containing protein -
PH192_RS25175 (PH192_25170) 84299..84751 + 453 WP_001326849.1 late promoter-activating protein -
PH192_RS25180 (PH192_25175) 84837..86030 + 1194 WP_032167084.1 terminase -
PH192_RS25185 (PH192_25180) 86030..87514 + 1485 WP_000124159.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..93185 93185


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13571.31 Da        Isoelectric Point: 5.6343

>T268544 WP_001216033.1 NZ_CP116245:82575-82955 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVAATLRRLYGSAE

Download         Length: 381 bp

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT268544 WP_001190712.1 NZ_CP116245:82354-82575 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZW91

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References