Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3354179..3354810 Replicon chromosome
Accession NZ_CP116241
Organism Escherichia coli strain 7.1994/NIST0056

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag PH192_RS16260 Protein ID WP_001259385.1
Coordinates 3354535..3354810 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag PH192_RS16255 Protein ID WP_000593555.1
Coordinates 3354179..3354538 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PH192_RS16230 (3350311) 3350311..3351255 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
PH192_RS16235 (3351252) 3351252..3352085 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
PH192_RS16240 (3352085) 3352085..3352849 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
PH192_RS16245 (3352846) 3352846..3353652 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
PH192_RS16250 (3353658) 3353658..3354059 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
PH192_RS16255 (3354179) 3354179..3354538 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
PH192_RS16260 (3354535) 3354535..3354810 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
PH192_RS16265 (3354883) 3354883..3356007 - 1125 WP_001314210.1 ABC transporter permease -
PH192_RS16270 (3356007) 3356007..3358742 - 2736 WP_000149152.1 ribosome-associated ATPase/putative transporter RbbA -
PH192_RS16275 (3358739) 3358739..3359806 - 1068 WP_000361471.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T268515 WP_001259385.1 NZ_CP116241:c3354810-3354535 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT268515 WP_000593555.1 NZ_CP116241:c3354538-3354179 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References