Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2701600..2702097 Replicon chromosome
Accession NZ_CP116241
Organism Escherichia coli strain 7.1994/NIST0056

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PH192_RS13185 Protein ID WP_001173343.1
Coordinates 2701600..2701773 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag PH192_RS13190 Protein ID WP_000398619.1
Coordinates 2701801..2702097 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PH192_RS13175 (2698270) 2698270..2699727 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
PH192_RS13180 (2699964) 2699964..2701481 + 1518 WP_001295074.1 lysine--tRNA ligase -
PH192_RS13185 (2701600) 2701600..2701773 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PH192_RS13190 (2701801) 2701801..2702097 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PH192_RS13195 (2702324) 2702324..2702596 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
PH192_RS13200 (2702608) 2702608..2702838 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
PH192_RS13205 (2703019) 2703019..2704650 + 1632 WP_001216487.1 sensor histidine kinase -
PH192_RS13210 (2704647) 2704647..2705366 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2629727..2702097 72370


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T268513 WP_001173343.1 NZ_CP116241:c2701773-2701600 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT268513 WP_000398619.1 NZ_CP116241:c2702097-2701801 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References