Detailed information of TA system TA026850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2176146..2176849
• Replicon: chromosome
• Accession: NC_017522
• Organism: Mycobacterium tuberculosis CCDC5180

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: CCDC5180_RS10145
• Protein ID: WP_003409778.1
• Coordinates: 2176146..2176475 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: CCDC5180_RS21375
• Protein ID: WP_003409780.1
• Coordinates: 2176472..2176849 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCDC5180_RS10125	2172529..2173599	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
CCDC5180_RS10130	2173596..2174111	+	516	WP_003409718.1	flavin reductase family protein	-
CCDC5180_RS10135	2174108..2175169	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
CCDC5180_RS10140	2175166..2175936	+	771	WP_003409775.1	SDR family oxidoreductase	-
CCDC5180_RS10145	2176146..2176475	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CCDC5180_RS21375	2176472..2176849	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
CCDC5180_RS10155	2176846..2177436	-	591	WP_003409784.1	SEC-C domain-containing protein	-
CCDC5180_RS10160	2177491..2178855	+	1365	WP_003903691.1	HNH endonuclease	-
CCDC5180_RS10165	2179010..2179462	-	453	WP_003899095.1	lipoprotein	-
CCDC5180_RS10170	2179526..2179927	+	402	WP_003409869.1	hypothetical protein	-
CCDC5180_RS10175	2179920..2180102	-	183	WP_003409870.1	hypothetical protein	-
CCDC5180_RS10180	2180216..2180566	-	351	WP_003409871.1	hypothetical protein	-
CCDC5180_RS10185	2180577..2181479	-	903	WP_003899097.1	hypothetical protein	-
CCDC5180_RS10190	2181500..2181691	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T26850 WP_003409778.1 NC_017522:c2176475-2176146 [Mycobacterium tuberculosis CCDC5180]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T26850 NC_017522:c2176475-2176146 [Mycobacterium tuberculosis CCDC5180]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT26850 WP_003409780.1 NC_017522:c2176849-2176472 [Mycobacterium tuberculosis CCDC5180]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT26850 NC_017522:c2176849-2176472 [Mycobacterium tuberculosis CCDC5180]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8