Detailed information of TA system TA268480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1274768..1275341
• Replicon: chromosome
• Accession: NZ_CP116224
• Organism: Actinomyces oris strain WVU627

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: PGE45_RS05515
• Protein ID: WP_271289377.1
• Coordinates: 1274994..1275341 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1Q8WC50
• Locus tag: PGE45_RS05510
• Protein ID: WP_075378155.1
• Coordinates: 1274768..1275007 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PGE45_RS05505 (PGE45_05505)	1271032..1274604	+	3573	WP_271289314.1	bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase	-
PGE45_RS05510 (PGE45_05510)	1274768..1275007	+	240	WP_075378155.1	antitoxin	Antitoxin
PGE45_RS05515 (PGE45_05515)	1274994..1275341	+	348	WP_271289377.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PGE45_RS05520 (PGE45_05520)	1275364..1275588	-	225	WP_020991564.1	helix-turn-helix transcriptional regulator	-
PGE45_RS05525 (PGE45_05525)	1275591..1276427	-	837	WP_271289315.1	ABC transporter permease	-
PGE45_RS05530 (PGE45_05530)	1276424..1277278	-	855	WP_271289316.1	ABC transporter ATP-binding protein	-
PGE45_RS05535 (PGE45_05535)	1277661..1279442	+	1782	WP_271289317.1	2-isopropylmalate synthase	-
PGE45_RS05540 (PGE45_05540)	1279465..1280214	+	750	WP_004565085.1	DNA repair protein RecO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12359.32 Da
• Isoelectric Point: 10.2914

>T268480 WP_271289377.1 NZ_CP116224:1274994-1275341 [Actinomyces oris]
MLRGEIRLVDFDPSVGSEANKRRPALIVSNDHANAAAARLGRGMVTVVPLTSSTSRVLPFQVFLPSEATGLPKDSKAQAE
QVRSIAVERVGEVVGWLPSGLMKMVNEALIQHLSL

Nucleotide

• Length: 348 bp

>T268480 NZ_CP116224:1274994-1275341 [Actinomyces oris]
ATGCTACGAGGTGAAATCCGCCTTGTCGATTTTGATCCTTCTGTCGGCAGTGAGGCGAACAAGCGTCGACCTGCGCTCAT
AGTGAGTAATGACCATGCCAATGCGGCGGCCGCGCGCCTTGGTCGTGGTATGGTGACAGTGGTCCCATTGACGAGCTCAA
CCTCGCGAGTTCTTCCCTTTCAGGTCTTCCTCCCCTCGGAGGCGACCGGCTTGCCCAAGGACTCCAAGGCTCAGGCGGAG
CAGGTTCGATCGATTGCTGTGGAGAGAGTCGGCGAGGTTGTCGGCTGGTTACCCAGTGGCCTGATGAAAATGGTTAATGA
GGCTCTGATCCAGCATCTCTCCTTGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8528.35 Da
• Isoelectric Point: 3.9937

>AT268480 WP_075378155.1 NZ_CP116224:1274768-1275007 [Actinomyces oris]
MKLSVSLSESDLILLDRCVERDGLASRSAGIQNAIRLLGKADLQDAYAEAWSSWDASEDATVWDSAVADGIDRGEDATR

Nucleotide

• Length: 240 bp

>AT268480 NZ_CP116224:1274768-1275007 [Actinomyces oris]
ATGAAGTTAAGCGTGAGTTTGTCGGAGAGTGACCTCATTCTGCTGGACCGATGCGTGGAGCGCGACGGTTTGGCGTCGAG
GTCTGCTGGTATTCAGAATGCCATCCGCCTGCTCGGAAAAGCTGATCTTCAGGATGCTTACGCTGAGGCCTGGTCCTCCT
GGGATGCCTCTGAGGATGCAACCGTCTGGGATAGTGCGGTCGCCGACGGGATCGATAGAGGTGAGGATGCTACGAGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8WC50