Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5013..5538 Replicon plasmid p2562_3
Accession NZ_CP116217
Organism Klebsiella michiganensis strain 2563

Toxin (Protein)


Gene name ccdB Uniprot ID A0A1D8K3Q5
Locus tag PHA56_RS31015 Protein ID WP_006788214.1
Coordinates 5013..5318 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag PHA56_RS31020 Protein ID WP_006788213.1
Coordinates 5320..5538 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PHA56_RS30990 (PHA56_30990) 19..894 + 876 WP_004098982.1 replication initiation protein -
PHA56_RS30995 (PHA56_30995) 1471..2097 + 627 WP_016946792.1 ParA family plasmid-partitioning AAA ATPase -
PHA56_RS31000 (PHA56_31000) 2094..2396 + 303 WP_004197636.1 hypothetical protein -
PHA56_RS31005 (PHA56_31005) 2812..3606 - 795 WP_016240356.1 site-specific integrase -
PHA56_RS31010 (PHA56_31010) 3804..4819 - 1016 Protein_4 hypothetical protein -
PHA56_RS31015 (PHA56_31015) 5013..5318 - 306 WP_006788214.1 type II toxin-antitoxin system toxin CcdB Toxin
PHA56_RS31020 (PHA56_31020) 5320..5538 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
PHA56_RS31025 (PHA56_31025) 6166..6396 + 231 WP_021312767.1 type II toxin-antitoxin system VapB family antitoxin -
PHA56_RS31030 (PHA56_31030) 6393..6809 + 417 WP_021312768.1 type II toxin-antitoxin system VapC family toxin -
PHA56_RS31035 (PHA56_31035) 6997..7701 - 705 WP_001067855.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..105902 105902


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11687.46 Da        Isoelectric Point: 7.2015

>T268466 WP_006788214.1 NZ_CP116217:c5318-5013 [Klebsiella michiganensis]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRLMTTDMASVPASVTGEEV
ADLRHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT268466 WP_006788213.1 NZ_CP116217:c5538-5320 [Klebsiella michiganensis]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8K3Q5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References