Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 54858..55594 Replicon plasmid p2562_2
Accession NZ_CP116216
Organism Klebsiella michiganensis strain 2563

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag PHA56_RS30305 Protein ID WP_003026803.1
Coordinates 54858..55340 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag PHA56_RS30310 Protein ID WP_003026799.1
Coordinates 55328..55594 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PHA56_RS30275 (PHA56_30275) 51471..52760 + 1290 WP_000922630.1 arsenite efflux transporter membrane subunit ArsB -
PHA56_RS30280 (PHA56_30280) 52773..53198 + 426 WP_000065758.1 glutaredoxin-dependent arsenate reductase -
PHA56_RS30285 (PHA56_30285) 53229..53495 - 267 WP_102047566.1 ArsA-related P-loop ATPase -
PHA56_RS30290 (PHA56_30290) 53569..54273 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
PHA56_RS30295 (PHA56_30295) 54328..54450 + 123 Protein_58 IS3 family transposase -
PHA56_RS30300 (PHA56_30300) 54472..54699 + 228 Protein_59 IS3 family transposase -
PHA56_RS30305 (PHA56_30305) 54858..55340 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
PHA56_RS30310 (PHA56_30310) 55328..55594 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
PHA56_RS30315 (PHA56_30315) 55864..56652 + 789 WP_077255422.1 hypothetical protein -
PHA56_RS30320 (PHA56_30320) 56598..57302 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
PHA56_RS30325 (PHA56_30325) 57366..60151 + 2786 Protein_64 Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..183276 183276
- inside IScluster/Tn - - 53569..71396 17827


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T268464 WP_003026803.1 NZ_CP116216:c55340-54858 [Klebsiella michiganensis]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT268464 WP_003026799.1 NZ_CP116216:c55594-55328 [Klebsiella michiganensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References