Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 227707..228510 Replicon plasmid p2562_1
Accession NZ_CP116215
Organism Klebsiella michiganensis strain 2563

Toxin (Protein)


Gene name KacT Uniprot ID A0A8B2S6X5
Locus tag PHA56_RS29320 Protein ID WP_048213819.1
Coordinates 227980..228510 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A8B2S700
Locus tag PHA56_RS29315 Protein ID WP_048213818.1
Coordinates 227707..227976 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PHA56_RS29290 (PHA56_29290) 222937..223284 - 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -
PHA56_RS29295 (PHA56_29295) 223284..223961 - 678 WP_106904188.1 IS66-like element accessory protein TnpA -
PHA56_RS29300 (PHA56_29300) 224048..224733 + 686 Protein_238 IS3 family transposase -
PHA56_RS29305 (PHA56_29305) 225406..226914 + 1509 WP_001189109.1 group II intron reverse transcriptase/maturase -
PHA56_RS29310 (PHA56_29310) 226995..227492 + 498 Protein_240 IS3 family transposase -
PHA56_RS29315 (PHA56_29315) 227707..227976 + 270 WP_048213818.1 DUF1778 domain-containing protein Antitoxin
PHA56_RS29320 (PHA56_29320) 227980..228510 + 531 WP_048213819.1 GNAT family N-acetyltransferase Toxin
PHA56_RS29325 (PHA56_29325) 228595..229599 - 1005 WP_048213820.1 hypothetical protein -
PHA56_RS29330 (PHA56_29330) 229604..229849 - 246 WP_048213821.1 hypothetical protein -
PHA56_RS29335 (PHA56_29335) 230608..231975 - 1368 WP_230324193.1 adenylate/guanylate cyclase domain-containing protein -
PHA56_RS29340 (PHA56_29340) 231985..232518 - 534 WP_048213822.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid formA - 1..362448 362448


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20322.17 Da        Isoelectric Point: 6.7544

>T268462 WP_048213819.1 NZ_CP116215:227980-228510 [Klebsiella michiganensis]
MDGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYLLVTREDKPRVMGYYTLSGSCFEKTLLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENSNSL
FYPTKSVEELFEVKDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10285.70 Da        Isoelectric Point: 5.7252

>AT268462 WP_048213818.1 NZ_CP116215:227707-227976 [Klebsiella michiganensis]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAITNPPVPNDRLKR
AANRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B2S6X5

Antitoxin

Source ID Structure
AlphaFold DB A0A8B2S700

References