Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2055293..2055883 Replicon chromosome
Accession NZ_CP116214
Organism Klebsiella michiganensis strain 2563

Toxin (Protein)


Gene name pemK Uniprot ID A0A0H3HDW1
Locus tag PHA56_RS09760 Protein ID WP_014229979.1
Coordinates 2055551..2055883 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag PHA56_RS09755 Protein ID WP_004852307.1
Coordinates 2055293..2055550 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PHA56_RS09730 (PHA56_09735) 2050549..2051154 - 606 WP_032750440.1 glutathione S-transferase family protein -
PHA56_RS09735 (PHA56_09740) 2051334..2052260 + 927 WP_014229983.1 LysR substrate-binding domain-containing protein -
PHA56_RS09740 (PHA56_09745) 2052298..2053296 - 999 WP_014229982.1 aldo/keto reductase -
PHA56_RS09745 (PHA56_09750) 2053397..2054311 + 915 WP_014229981.1 LysR family transcriptional regulator -
PHA56_RS09750 (PHA56_09755) 2054502..2054582 - 81 Protein_1911 molybdopterin-binding protein -
PHA56_RS09755 (PHA56_09760) 2055293..2055550 + 258 WP_004852307.1 antitoxin Antitoxin
PHA56_RS09760 (PHA56_09765) 2055551..2055883 + 333 WP_014229979.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
PHA56_RS09770 (PHA56_09775) 2056206..2057663 + 1458 WP_014229916.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
PHA56_RS09780 (PHA56_09785) 2058626..2060080 - 1455 WP_014229909.1 AMP nucleosidase -
PHA56_RS09785 (PHA56_09790) 2060213..2060470 - 258 WP_014229908.1 histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2045586..2055883 10297


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11740.57 Da        Isoelectric Point: 10.1863

>T268453 WP_014229979.1 NZ_CP116214:2055551-2055883 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT268453 WP_004852307.1 NZ_CP116214:2055293-2055550 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3HDW1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References