268411

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 96036..96679 Replicon plasmid pDETEC3
Accession NZ_CP116195
Organism Escherichia coli strain DETEC-E1005

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag PIB63_RS24760 Protein ID WP_001034046.1
Coordinates 96263..96679 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag PIB63_RS24755 Protein ID WP_001261278.1
Coordinates 96036..96266 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIB63_RS24725 (PIB63_24725) 91547..91852 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
PIB63_RS24730 (PIB63_24730) 91854..92072 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
PIB63_RS24735 (PIB63_24735) 92663..93151 + 489 WP_011254646.1 hypothetical protein -
PIB63_RS24740 (PIB63_24740) 93185..94318 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
PIB63_RS24745 (PIB63_24745) 94485..95258 - 774 WP_000905949.1 hypothetical protein -
PIB63_RS24750 (PIB63_24750) 95271..95771 - 501 WP_001773886.1 HEPN family nuclease -
PIB63_RS24755 (PIB63_24755) 96036..96266 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
PIB63_RS24760 (PIB63_24760) 96263..96679 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
PIB63_RS24765 (PIB63_24765) 96724..100518 - 3795 WP_001144732.1 hypothetical protein -
PIB63_RS24770 (PIB63_24770) 100899..101129 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
PIB63_RS24775 (PIB63_24775) 101126..101542 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / blaTEM-1B / tet(B) / dfrA17 / sul2 / aph(3'')-Ib / aph(6)-Id senB 1..108065 108065


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T268411 WP_001034046.1 NZ_CP116195:96263-96679 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT268411 WP_001261278.1 NZ_CP116195:96036-96266 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References