Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4427003..4427500 Replicon chromosome
Accession NZ_CP116188
Organism Escherichia coli strain DETEC-E223

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PIC88_RS21720 Protein ID WP_001173343.1
Coordinates 4427003..4427176 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag PIC88_RS21725 Protein ID WP_000398619.1
Coordinates 4427204..4427500 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC88_RS21710 (4423673) 4423673..4425130 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
PIC88_RS21715 (4425367) 4425367..4426884 + 1518 WP_001295074.1 lysine--tRNA ligase -
PIC88_RS21720 (4427003) 4427003..4427176 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PIC88_RS21725 (4427204) 4427204..4427500 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PIC88_RS21730 (4427727) 4427727..4427999 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
PIC88_RS21735 (4428011) 4428011..4428249 - 239 Protein_4253 4Fe-4S mono-cluster protein YjdI -
PIC88_RS21740 (4428430) 4428430..4430061 + 1632 WP_001216477.1 sensor histidine kinase -
PIC88_RS21745 (4430058) 4430058..4430777 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T268352 WP_001173343.1 NZ_CP116188:c4427176-4427003 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT268352 WP_000398619.1 NZ_CP116188:c4427500-4427204 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References