Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3846937..3847591 Replicon chromosome
Accession NZ_CP116188
Organism Escherichia coli strain DETEC-E223

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag PIC88_RS18760 Protein ID WP_000244781.1
Coordinates 3846937..3847344 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag PIC88_RS18765 Protein ID WP_000354046.1
Coordinates 3847325..3847591 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC88_RS18740 (3842894) 3842894..3844627 - 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -
PIC88_RS18745 (3844633) 3844633..3845343 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
PIC88_RS18750 (3845368) 3845368..3846264 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
PIC88_RS18755 (3846376) 3846376..3846897 + 522 WP_001055874.1 flavodoxin FldB -
PIC88_RS18760 (3846937) 3846937..3847344 - 408 WP_000244781.1 protein YgfX Toxin
PIC88_RS18765 (3847325) 3847325..3847591 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
PIC88_RS18770 (3847834) 3847834..3848814 + 981 WP_000886083.1 tRNA-modifying protein YgfZ -
PIC88_RS18775 (3849010) 3849010..3849669 - 660 WP_000250274.1 hemolysin III family protein -
PIC88_RS18780 (3849833) 3849833..3850144 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
PIC88_RS18785 (3850189) 3850189..3851622 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
PIC88_RS18790 (3851679) 3851679..3852422 - 744 WP_061359834.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T268346 WP_000244781.1 NZ_CP116188:c3847344-3846937 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT268346 WP_000354046.1 NZ_CP116188:c3847591-3847325 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References