Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4161686..4162262 Replicon chromosome
Accession NZ_CP116181
Organism Escherichia coli strain DETEC-E480

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag PIB86_RS20440 Protein ID WP_001295743.1
Coordinates 4161975..4162262 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag PIB86_RS20435 Protein ID WP_000063148.1
Coordinates 4161686..4161988 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIB86_RS20420 (4158282) 4158282..4160432 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
PIB86_RS20425 (4160482) 4160482..4160685 + 204 WP_000467859.1 YbdD/YjiX family protein -
PIB86_RS20430 (4160696) 4160696..4161652 + 957 WP_001295745.1 GTPase -
PIB86_RS20435 (4161686) 4161686..4161988 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
PIB86_RS20440 (4161975) 4161975..4162262 - 288 WP_001295743.1 BrnT family toxin Toxin
PIB86_RS20445 (4162459) 4162459..4163625 + 1167 WP_000800831.1 restriction endonuclease -
PIB86_RS20450 (4163689) 4163689..4165308 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
PIB86_RS20455 (4165298) 4165298..4166602 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T268319 WP_001295743.1 NZ_CP116181:c4162262-4161975 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT268319 WP_000063148.1 NZ_CP116181:c4161988-4161686 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References