Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 292423..293223 Replicon chromosome
Accession NZ_CP116181
Organism Escherichia coli strain DETEC-E480

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag PIB86_RS01370 Protein ID WP_000342452.1
Coordinates 292696..293223 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag PIB86_RS01365 Protein ID WP_001277107.1
Coordinates 292423..292689 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIB86_RS01345 (288082) 288082..288750 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
PIB86_RS01350 (288743) 288743..289801 + 1059 WP_001042013.1 permease-like cell division protein FtsX -
PIB86_RS01355 (290046) 290046..290900 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
PIB86_RS01360 (291171) 291171..292274 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
PIB86_RS01365 (292423) 292423..292689 + 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
PIB86_RS01370 (292696) 292696..293223 + 528 WP_000342452.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
PIB86_RS01375 (293220) 293220..293603 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
PIB86_RS01380 (294026) 294026..295135 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
PIB86_RS01385 (295183) 295183..296109 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
PIB86_RS01390 (296106) 296106..297383 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
PIB86_RS01395 (297380) 297380..298147 + 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T268304 WP_000342452.1 NZ_CP116181:292696-293223 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT268304 WP_001277107.1 NZ_CP116181:292423-292689 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References