Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3664704..3665287 Replicon chromosome
Accession NZ_CP116176
Organism Escherichia coli strain DETEC-E601

Toxin (Protein)


Gene name mazF Uniprot ID F4NJF2
Locus tag PIC02_RS17745 Protein ID WP_000254731.1
Coordinates 3664704..3665039 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag PIC02_RS17750 Protein ID WP_000581937.1
Coordinates 3665039..3665287 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC02_RS17730 (3660591) 3660591..3661889 - 1299 WP_000036723.1 phosphopyruvate hydratase -
PIC02_RS17735 (3661977) 3661977..3663614 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
PIC02_RS17740 (3663842) 3663842..3664633 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
PIC02_RS17745 (3664704) 3664704..3665039 - 336 WP_000254731.1 endoribonuclease MazF Toxin
PIC02_RS17750 (3665039) 3665039..3665287 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
PIC02_RS17755 (3665365) 3665365..3667599 - 2235 WP_000226815.1 GTP pyrophosphokinase -
PIC02_RS17760 (3667647) 3667647..3668948 - 1302 WP_000046789.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12043.02 Da        Isoelectric Point: 8.1170

>T268295 WP_000254731.1 NZ_CP116176:c3665039-3664704 [Escherichia coli]
MVSRYVPDMGDLICVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVVPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT268295 WP_000581937.1 NZ_CP116176:c3665287-3665039 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F4NJF2

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References