Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1147016..1147853 Replicon chromosome
Accession NZ_CP116176
Organism Escherichia coli strain DETEC-E601

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag PIC02_RS05435 Protein ID WP_000227784.1
Coordinates 1147016..1147558 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag PIC02_RS05440 Protein ID WP_001297137.1
Coordinates 1147542..1147853 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC02_RS05415 (1142555) 1142555..1143466 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
PIC02_RS05420 (1143634) 1143634..1144125 + 492 WP_001138904.1 nucleotide binding protein YajQ -
PIC02_RS05425 (1144253) 1144253..1145617 - 1365 WP_001000960.1 MFS transporter -
PIC02_RS05430 (1146025) 1146025..1146960 + 936 Protein_1057 tetratricopeptide repeat protein -
PIC02_RS05435 (1147016) 1147016..1147558 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
PIC02_RS05440 (1147542) 1147542..1147853 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
PIC02_RS05445 (1148038) 1148038..1148928 - 891 WP_000971336.1 heme o synthase -
PIC02_RS05450 (1148940) 1148940..1149269 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
PIC02_RS05455 (1149269) 1149269..1149883 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
PIC02_RS05460 (1149873) 1149873..1151864 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
PIC02_RS05465 (1151886) 1151886..1152833 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T268286 WP_000227784.1 NZ_CP116176:c1147558-1147016 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT268286 WP_001297137.1 NZ_CP116176:c1147853-1147542 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References