Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 277667..278164 Replicon chromosome
Accession NZ_CP116176
Organism Escherichia coli strain DETEC-E601

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PIC02_RS01280 Protein ID WP_001173343.1
Coordinates 277991..278164 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag PIC02_RS01275 Protein ID WP_000398619.1
Coordinates 277667..277963 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC02_RS01255 (274398) 274398..275117 - 720 WP_000611288.1 two-component system response regulator DcuR -
PIC02_RS01260 (275114) 275114..276745 - 1632 WP_001216461.1 sensor histidine kinase -
PIC02_RS01265 (276926) 276926..277156 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
PIC02_RS01270 (277168) 277168..277440 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
PIC02_RS01275 (277667) 277667..277963 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PIC02_RS01280 (277991) 277991..278164 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PIC02_RS01285 (278283) 278283..279800 - 1518 WP_001295074.1 lysine--tRNA ligase -
PIC02_RS01290 (280037) 280037..281494 - 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T268284 WP_001173343.1 NZ_CP116176:277991-278164 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT268284 WP_000398619.1 NZ_CP116176:277667-277963 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References