Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4321245..4321742 Replicon chromosome
Accession NZ_CP116169
Organism Escherichia coli strain DETEC-P1056

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PIC86_RS20935 Protein ID WP_001173343.1
Coordinates 4321245..4321418 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag PIC86_RS20940 Protein ID WP_000398619.1
Coordinates 4321446..4321742 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC86_RS20925 (4317915) 4317915..4319372 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
PIC86_RS20930 (4319609) 4319609..4321126 + 1518 WP_001295074.1 lysine--tRNA ligase -
PIC86_RS20935 (4321245) 4321245..4321418 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PIC86_RS20940 (4321446) 4321446..4321742 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PIC86_RS20945 (4321969) 4321969..4322241 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
PIC86_RS20950 (4322253) 4322253..4322483 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
PIC86_RS20955 (4322664) 4322664..4324295 + 1632 WP_001216461.1 sensor histidine kinase -
PIC86_RS20960 (4324292) 4324292..4325011 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T268277 WP_001173343.1 NZ_CP116169:c4321418-4321245 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT268277 WP_000398619.1 NZ_CP116169:c4321742-4321446 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References