268273

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3494553..3495390 Replicon chromosome
Accession NZ_CP116169
Organism Escherichia coli strain DETEC-P1056

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag PIC86_RS17035 Protein ID WP_000227784.1
Coordinates 3494848..3495390 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag PIC86_RS17030 Protein ID WP_001297137.1
Coordinates 3494553..3494864 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC86_RS17005 (3489573) 3489573..3490520 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
PIC86_RS17010 (3490542) 3490542..3492533 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
PIC86_RS17015 (3492523) 3492523..3493137 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
PIC86_RS17020 (3493137) 3493137..3493466 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
PIC86_RS17025 (3493478) 3493478..3494368 + 891 WP_000971336.1 heme o synthase -
PIC86_RS17030 (3494553) 3494553..3494864 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
PIC86_RS17035 (3494848) 3494848..3495390 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
PIC86_RS17040 (3495446) 3495446..3496381 - 936 WP_001297127.1 tetratricopeptide repeat protein -
PIC86_RS17045 (3496789) 3496789..3498153 + 1365 WP_001000978.1 MFS transporter -
PIC86_RS17050 (3498281) 3498281..3498772 - 492 WP_001138904.1 nucleotide binding protein YajQ -
PIC86_RS17055 (3498940) 3498940..3499851 + 912 WP_000705847.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T268273 WP_000227784.1 NZ_CP116169:3494848-3495390 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT268273 WP_001297137.1 NZ_CP116169:3494553-3494864 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References