Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1016603..1017186 Replicon chromosome
Accession NZ_CP116169
Organism Escherichia coli strain DETEC-P1056

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag PIC86_RS04915 Protein ID WP_000254745.1
Coordinates 1016851..1017186 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag PIC86_RS04910 Protein ID WP_000581937.1
Coordinates 1016603..1016851 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC86_RS04900 (1012942) 1012942..1014243 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
PIC86_RS04905 (1014291) 1014291..1016525 + 2235 WP_000226815.1 GTP pyrophosphokinase -
PIC86_RS04910 (1016603) 1016603..1016851 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
PIC86_RS04915 (1016851) 1016851..1017186 + 336 WP_000254745.1 endoribonuclease MazF Toxin
PIC86_RS04920 (1017257) 1017257..1018048 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
PIC86_RS04925 (1018276) 1018276..1019913 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
PIC86_RS04930 (1020001) 1020001..1021299 + 1299 WP_000036723.1 phosphopyruvate hydratase -
PIC86_RS04935 (1021355) 1021355..1021717 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T268262 WP_000254745.1 NZ_CP116169:1016851-1017186 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT268262 WP_000581937.1 NZ_CP116169:1016603-1016851 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References