Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 286631..287431 Replicon chromosome
Accession NZ_CP116169
Organism Escherichia coli strain DETEC-P1056

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag PIC86_RS01305 Protein ID WP_000342449.1
Coordinates 286904..287431 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag PIC86_RS01300 Protein ID WP_001277108.1
Coordinates 286631..286897 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC86_RS01280 (282289) 282289..282957 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
PIC86_RS01285 (282950) 282950..284008 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
PIC86_RS01290 (284253) 284253..285107 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
PIC86_RS01295 (285378) 285378..286481 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
PIC86_RS01300 (286631) 286631..286897 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
PIC86_RS01305 (286904) 286904..287431 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
PIC86_RS01310 (287428) 287428..287811 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
PIC86_RS01315 (288235) 288235..289344 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
PIC86_RS01320 (289392) 289392..290318 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
PIC86_RS01325 (290315) 290315..291592 + 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
PIC86_RS01330 (291589) 291589..292356 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T268259 WP_000342449.1 NZ_CP116169:286904-287431 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT268259 WP_001277108.1 NZ_CP116169:286631-286897 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References