Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4256192..4256689 Replicon chromosome
Accession NZ_CP116166
Organism Escherichia coli strain DETEC-P169

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PID12_RS20900 Protein ID WP_001173343.1
Coordinates 4256192..4256365 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag PID12_RS20905 Protein ID WP_001425728.1
Coordinates 4256393..4256689 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PID12_RS20890 (4252862) 4252862..4254319 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
PID12_RS20895 (4254556) 4254556..4256073 + 1518 WP_001295074.1 lysine--tRNA ligase -
PID12_RS20900 (4256192) 4256192..4256365 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PID12_RS20905 (4256393) 4256393..4256689 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PID12_RS20910 (4256916) 4256916..4257188 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
PID12_RS20915 (4257200) 4257200..4257430 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
PID12_RS20920 (4257611) 4257611..4259242 + 1632 WP_001216487.1 sensor histidine kinase -
PID12_RS20925 (4259239) 4259239..4259958 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T268247 WP_001173343.1 NZ_CP116166:c4256365-4256192 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT268247 WP_001425728.1 NZ_CP116166:c4256689-4256393 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References