Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 318407..319207 Replicon chromosome
Accession NZ_CP116166
Organism Escherichia coli strain DETEC-P169

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag PID12_RS01470 Protein ID WP_000342449.1
Coordinates 318680..319207 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag PID12_RS01465 Protein ID WP_001277108.1
Coordinates 318407..318673 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PID12_RS01445 (314064) 314064..314732 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
PID12_RS01450 (314725) 314725..315783 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
PID12_RS01455 (316028) 316028..316882 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
PID12_RS01460 (317154) 317154..318257 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
PID12_RS01465 (318407) 318407..318673 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
PID12_RS01470 (318680) 318680..319207 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
PID12_RS01475 (319204) 319204..319587 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
PID12_RS01480 (320011) 320011..321120 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
PID12_RS01485 (321168) 321168..322094 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
PID12_RS01490 (322091) 322091..323368 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
PID12_RS01495 (323365) 323365..324132 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T268233 WP_000342449.1 NZ_CP116166:318680-319207 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT268233 WP_001277108.1 NZ_CP116166:318407-318673 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References