Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4285419..4285916 Replicon chromosome
Accession NZ_CP116159
Organism Escherichia coli strain DETEC-P351

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PIC26_RS20720 Protein ID WP_001173343.1
Coordinates 4285419..4285592 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag PIC26_RS20725 Protein ID WP_000398619.1
Coordinates 4285620..4285916 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC26_RS20710 (4282089) 4282089..4283546 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
PIC26_RS20715 (4283783) 4283783..4285300 + 1518 WP_001295074.1 lysine--tRNA ligase -
PIC26_RS20720 (4285419) 4285419..4285592 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PIC26_RS20725 (4285620) 4285620..4285916 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PIC26_RS20730 (4286143) 4286143..4286415 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
PIC26_RS20735 (4286427) 4286427..4286657 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
PIC26_RS20740 (4286838) 4286838..4288469 + 1632 WP_001216477.1 sensor histidine kinase -
PIC26_RS20745 (4288466) 4288466..4289185 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T268221 WP_001173343.1 NZ_CP116159:c4285592-4285419 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT268221 WP_000398619.1 NZ_CP116159:c4285916-4285620 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References