Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4067813..4068389 Replicon chromosome
Accession NZ_CP116159
Organism Escherichia coli strain DETEC-P351

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag PIC26_RS19580 Protein ID WP_001297643.1
Coordinates 4068102..4068389 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag PIC26_RS19575 Protein ID WP_000063160.1
Coordinates 4067813..4068115 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC26_RS19560 (4064409) 4064409..4066559 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
PIC26_RS19565 (4066609) 4066609..4066812 + 204 WP_000467859.1 YbdD/YjiX family protein -
PIC26_RS19570 (4066823) 4066823..4067779 + 957 WP_001297640.1 GTPase -
PIC26_RS19575 (4067813) 4067813..4068115 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
PIC26_RS19580 (4068102) 4068102..4068389 - 288 WP_001297643.1 BrnT family toxin Toxin
PIC26_RS19585 (4068588) 4068588..4069284 - 697 Protein_3828 IS1 family transposase -
PIC26_RS19590 (4069599) 4069599..4070831 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
PIC26_RS19595 (4070872) 4070872..4072152 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4068588..4068797 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T268219 WP_001297643.1 NZ_CP116159:c4068389-4068102 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT268219 WP_000063160.1 NZ_CP116159:c4068115-4067813 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References