Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3964483..3965059 Replicon chromosome
Accession NZ_CP116152
Organism Escherichia coli strain DETEC-P61

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag PIC71_RS19680 Protein ID WP_001297643.1
Coordinates 3964772..3965059 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag PIC71_RS19675 Protein ID WP_000063160.1
Coordinates 3964483..3964785 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC71_RS19660 (3961079) 3961079..3963229 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
PIC71_RS19665 (3963279) 3963279..3963482 + 204 WP_000467859.1 YbdD/YjiX family protein -
PIC71_RS19670 (3963493) 3963493..3964449 + 957 WP_001297640.1 GTPase -
PIC71_RS19675 (3964483) 3964483..3964785 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
PIC71_RS19680 (3964772) 3964772..3965059 - 288 WP_001297643.1 BrnT family toxin Toxin
PIC71_RS19685 (3965258) 3965258..3965954 - 697 Protein_3847 IS1 family transposase -
PIC71_RS19690 (3966269) 3966269..3967501 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
PIC71_RS19695 (3967542) 3967542..3968822 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3965258..3965467 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T268191 WP_001297643.1 NZ_CP116152:c3965059-3964772 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT268191 WP_000063160.1 NZ_CP116152:c3964785-3964483 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References