Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4518509..4519006 Replicon chromosome
Accession NZ_CP116136
Organism Escherichia coli strain DETEC-P649

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag PIB66_RS21515 Protein ID WP_001173343.1
Coordinates 4518509..4518682 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag PIB66_RS21520 Protein ID WP_000398619.1
Coordinates 4518710..4519006 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIB66_RS21505 (4515179) 4515179..4516636 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
PIB66_RS21510 (4516873) 4516873..4518390 + 1518 WP_001550472.1 lysine--tRNA ligase -
PIB66_RS21515 (4518509) 4518509..4518682 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
PIB66_RS21520 (4518710) 4518710..4519006 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
PIB66_RS21525 (4519235) 4519235..4519507 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
PIB66_RS21530 (4519519) 4519519..4519749 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
PIB66_RS21535 (4519929) 4519929..4521560 + 1632 WP_001216477.1 sensor histidine kinase -
PIB66_RS21540 (4521557) 4521557..4522276 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4494597..4519006 24409


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T268139 WP_001173343.1 NZ_CP116136:c4518682-4518509 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT268139 WP_000398619.1 NZ_CP116136:c4519006-4518710 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References