Detailed information of TA system TA268134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3877992..3879681
• Replicon: chromosome
• Accession: NZ_CP116136
• Organism: Escherichia coli strain DETEC-P649

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: V0Z9M0
• Locus tag: PIB66_RS18455
• Protein ID: WP_000365809.1
• Coordinates: 3877992..3878912 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: PIB66_RS18460
• Protein ID: WP_001550661.1
• Coordinates: 3878899..3879681 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PIB66_RS18440 (3874082)	3874082..3875209	-	1128	WP_001550663.1	MFS transporter	-
PIB66_RS18445 (3875351)	3875351..3876409	-	1059	WP_122987197.1	alpha/beta hydrolase	-
PIB66_RS18450 (3876674)	3876674..3877558	+	885	WP_001119037.1	LysR family transcriptional regulator	-
PIB66_RS18455 (3877992)	3877992..3878912	-	921	WP_000365809.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
PIB66_RS18460 (3878899)	3878899..3879681	-	783	WP_001550661.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
PIB66_RS18465 (3879997)	3879997..3883206	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
PIB66_RS18470 (3883553)	3883553..3884023	+	471	WP_000201185.1	hypothetical protein	-
PIB66_RS18475 (3884020)	3884020..3884122	+	103	Protein_3617	helix-turn-helix domain-containing protein	-
PIB66_RS18480 (3884214)	3884214..3884372	+	159	Protein_3618	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34571.01 Da
• Isoelectric Point: 7.2649

>T268134 WP_000365809.1 NZ_CP116136:c3878912-3877992 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29191.43 Da
• Isoelectric Point: 8.9059

>AT268134 WP_001550661.1 NZ_CP116136:c3879681-3878899 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: V0Z9M0