Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3012964..3013748 Replicon chromosome
Accession NZ_CP116136
Organism Escherichia coli strain DETEC-P649

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag PIB66_RS14510 Protein ID WP_000613626.1
Coordinates 3013254..3013748 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag PIB66_RS14505 Protein ID WP_001110447.1
Coordinates 3012964..3013257 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIB66_RS14495 (3008114) 3008114..3009073 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
PIB66_RS14500 (3009646) 3009646..3012831 + 3186 WP_001550951.1 ribonuclease E -
PIB66_RS14505 (3012964) 3012964..3013257 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
PIB66_RS14510 (3013254) 3013254..3013748 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
PIB66_RS14515 (3013843) 3013843..3014796 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
PIB66_RS14520 (3014808) 3014808..3016451 - 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
PIB66_RS14525 (3016517) 3016517..3017458 - 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
PIB66_RS14530 (3017458) 3017458..3018555 - 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T268131 WP_000613626.1 NZ_CP116136:3013254-3013748 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT268131 WP_001110447.1 NZ_CP116136:3012964-3013257 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References