Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1002399..1003053 Replicon chromosome
Accession NZ_CP116136
Organism Escherichia coli strain DETEC-P649

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag PIB66_RS04895 Protein ID WP_000244781.1
Coordinates 1002646..1003053 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag PIB66_RS04890 Protein ID WP_000354046.1
Coordinates 1002399..1002665 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIB66_RS04870 (998487) 998487..999920 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
PIB66_RS04875 (999965) 999965..1000276 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
PIB66_RS04880 (1000440) 1000440..1001099 + 660 WP_000250274.1 hemolysin III family protein -
PIB66_RS04885 (1001176) 1001176..1002156 - 981 WP_000886079.1 tRNA-modifying protein YgfZ -
PIB66_RS04890 (1002399) 1002399..1002665 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
PIB66_RS04895 (1002646) 1002646..1003053 + 408 WP_000244781.1 protein YgfX Toxin
PIB66_RS04900 (1003093) 1003093..1003614 - 522 WP_001055874.1 flavodoxin FldB -
PIB66_RS04905 (1003726) 1003726..1004622 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
PIB66_RS04910 (1004647) 1004647..1005357 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
PIB66_RS04915 (1005363) 1005363..1007096 + 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T268122 WP_000244781.1 NZ_CP116136:1002646-1003053 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT268122 WP_000354046.1 NZ_CP116136:1002399-1002665 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References