Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3012955..3013739 Replicon chromosome
Accession NZ_CP116123
Organism Escherichia coli strain DETEC-P666

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag PIB57_RS14515 Protein ID WP_000613626.1
Coordinates 3013245..3013739 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag PIB57_RS14510 Protein ID WP_001110447.1
Coordinates 3012955..3013248 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIB57_RS14500 (3008105) 3008105..3009064 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
PIB57_RS14505 (3009637) 3009637..3012822 + 3186 WP_001550951.1 ribonuclease E -
PIB57_RS14510 (3012955) 3012955..3013248 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
PIB57_RS14515 (3013245) 3013245..3013739 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
PIB57_RS14520 (3013834) 3013834..3014787 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
PIB57_RS14525 (3014799) 3014799..3016442 - 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
PIB57_RS14530 (3016508) 3016508..3017449 - 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
PIB57_RS14535 (3017449) 3017449..3018546 - 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T268098 WP_000613626.1 NZ_CP116123:3013245-3013739 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT268098 WP_001110447.1 NZ_CP116123:3012955-3013248 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References