Detailed information of TA system TA026806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-toxA/Tad-HTH_37
• Location: 462342..462970
• Replicon: chromosome
• Accession: NC_017473
• Organism: Lactobacillus casei LC2W

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A806L3G4
• Locus tag: LC2W_RS02310
• Protein ID: WP_012490993.1
• Coordinates: 462602..462970 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: LC2W_RS02305
• Protein ID: WP_003583264.1
• Coordinates: 462342..462602 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LC2W_RS02280	458233..458976	+	744	WP_012490990.1	SDR family oxidoreductase	-
LC2W_RS02285	459089..459715	+	627	WP_003577713.1	flavodoxin	-
LC2W_RS02290	459966..460469	+	504	WP_012490991.1	TetR/AcrR family transcriptional regulator	-
LC2W_RS02295	460474..461535	+	1062	WP_003563374.1	ABC transporter permease	-
LC2W_RS02300	461540..462229	+	690	WP_012490992.1	ABC transporter ATP-binding protein	-
LC2W_RS02305	462342..462602	-	261	WP_003583264.1	helix-turn-helix transcriptional regulator	Antitoxin
LC2W_RS02310	462602..462970	-	369	WP_012490993.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LC2W_RS02315	463261..464631	-	1371	WP_012490994.1	FAD-dependent oxidoreductase	-
LC2W_RS02320	464995..465813	-	819	WP_012490995.1	Cof-type HAD-IIB family hydrolase	-
LC2W_RS02325	466129..466398	-	270	WP_012490996.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
LC2W_RS02330	466639..467646	+	1008	WP_003563383.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (34-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-79)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14970.02 Da
• Isoelectric Point: 8.3986

>T26806 WP_012490993.1 NC_017473:c462970-462602 [Lacticaseibacillus paracasei]
MYKIDFYEDRDGYSEIEDFLDQLRHSHQKNNVSLLNKITKELYMLQNLGPRLREPHAKFLKGYAYPIMELRPMQERIFYA
AWQKDHFVLLHHYTKKTNKTDHREILRAQSNLEDWLSRKEEN

Nucleotide

• Length: 369 bp

>T26806 NC_017473:c462970-462602 [Lacticaseibacillus paracasei]
ATGTATAAAATTGACTTCTATGAGGATCGCGATGGCTACTCTGAGATCGAAGACTTTCTTGATCAGTTACGCCATAGTCA
TCAAAAAAATAATGTGAGCCTTTTAAACAAAATCACAAAGGAACTCTACATGCTGCAAAATCTTGGTCCGCGCTTACGTG
AACCTCACGCGAAGTTTCTGAAGGGATATGCTTATCCGATCATGGAATTGCGTCCCATGCAAGAAAGAATCTTTTATGCT
GCATGGCAAAAGGATCATTTCGTTTTGCTTCATCACTACACCAAGAAAACAAACAAAACTGATCACAGAGAAATTTTACG
GGCACAGTCTAACCTCGAAGATTGGCTCTCTAGAAAGGAAGAAAATTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9828.45 Da
• Isoelectric Point: 10.2439

>AT26806 WP_003583264.1 NC_017473:c462602-462342 [Lacticaseibacillus paracasei]
MTTWEDFSQKLKKDNPEKLAIIKTLADLESERIKQGISQKDFAEKIGMKQPQLAKIERLDSLPTLATLNRYARGLGKEIR
LSIVTQ

Nucleotide

• Length: 261 bp

>AT26806 NC_017473:c462602-462342 [Lacticaseibacillus paracasei]
ATGACGACATGGGAAGACTTCAGTCAGAAACTGAAGAAGGACAATCCAGAAAAGTTGGCAATCATCAAAACCTTGGCCGA
TTTAGAAAGTGAACGAATCAAGCAAGGAATCTCTCAAAAGGACTTTGCAGAAAAAATTGGTATGAAACAGCCGCAACTAG
CAAAGATTGAGCGCTTAGACTCTCTTCCGACTCTGGCAACACTCAATCGATACGCGAGAGGGCTTGGAAAAGAAATTCGT
TTATCAATTGTTACTCAGTGA

Structures

Toxin

• AlphaFold DB: A0A806L3G4