Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3962992..3963568 Replicon chromosome
Accession NZ_CP116110
Organism Escherichia coli strain DETEC-P80

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag PIC84_RS19520 Protein ID WP_001297643.1
Coordinates 3963281..3963568 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag PIC84_RS19515 Protein ID WP_000063156.1
Coordinates 3962992..3963294 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC84_RS19500 (3959588) 3959588..3961738 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
PIC84_RS19505 (3961788) 3961788..3961991 + 204 WP_000467859.1 YbdD/YjiX family protein -
PIC84_RS19510 (3962002) 3962002..3962958 + 957 WP_001297640.1 GTPase -
PIC84_RS19515 (3962992) 3962992..3963294 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
PIC84_RS19520 (3963281) 3963281..3963568 - 288 WP_001297643.1 BrnT family toxin Toxin
PIC84_RS19525 (3963767) 3963767..3964464 - 698 Protein_3815 IS1 family transposase -
PIC84_RS19530 (3964521) 3964521..3964616 + 96 Protein_3816 dienelactone hydrolase family protein -
PIC84_RS19535 (3964762) 3964762..3965994 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
PIC84_RS19540 (3966035) 3966035..3967315 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3963767..3963976 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T268035 WP_001297643.1 NZ_CP116110:c3963568-3963281 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT268035 WP_000063156.1 NZ_CP116110:c3963294-3962992 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References