Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 291374..292174 Replicon chromosome
Accession NZ_CP116106
Organism Escherichia coli strain DETEC-P829

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag PIC05_RS01360 Protein ID WP_000342449.1
Coordinates 291647..292174 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag PIC05_RS01355 Protein ID WP_001277108.1
Coordinates 291374..291640 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC05_RS01335 (287032) 287032..287700 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
PIC05_RS01340 (287693) 287693..288751 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
PIC05_RS01345 (288996) 288996..289850 + 855 WP_000130221.1 RNA polymerase sigma factor RpoH -
PIC05_RS01350 (290121) 290121..291224 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
PIC05_RS01355 (291374) 291374..291640 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
PIC05_RS01360 (291647) 291647..292174 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
PIC05_RS01365 (292171) 292171..292554 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
PIC05_RS01370 (292978) 292978..294087 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
PIC05_RS01375 (294135) 294135..295061 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
PIC05_RS01380 (295058) 295058..296335 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
PIC05_RS01385 (296332) 296332..297099 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T267985 WP_000342449.1 NZ_CP116106:291647-292174 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT267985 WP_001277108.1 NZ_CP116106:291374-291640 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References