Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 505039..505615 Replicon chromosome
Accession NZ_CP116094
Organism Escherichia coli strain DETEC-S560

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag PIC78_RS02460 Protein ID WP_001297643.1
Coordinates 505039..505326 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag PIC78_RS02465 Protein ID WP_000063160.1
Coordinates 505313..505615 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC78_RS02445 (501275) 501275..502555 - 1281 WP_001037390.1 DUF445 domain-containing protein -
PIC78_RS02450 (502596) 502596..503828 - 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
PIC78_RS02455 (504143) 504143..504840 + 698 Protein_473 IS1 family transposase -
PIC78_RS02460 (505039) 505039..505326 + 288 WP_001297643.1 BrnT family toxin Toxin
PIC78_RS02465 (505313) 505313..505615 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
PIC78_RS02470 (505649) 505649..506605 - 957 WP_001297640.1 GTPase -
PIC78_RS02475 (506616) 506616..506819 - 204 WP_000467859.1 YbdD/YjiX family protein -
PIC78_RS02480 (506869) 506869..509019 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T267897 WP_001297643.1 NZ_CP116094:505039-505326 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT267897 WP_000063160.1 NZ_CP116094:505313-505615 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References