Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 12242..12885 Replicon plasmid pDETEC72
Accession NZ_CP116089
Organism Escherichia coli strain DETEC-S565

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag PIC22_RS23485 Protein ID WP_001034044.1
Coordinates 12469..12885 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag PIC22_RS23480 Protein ID WP_001261286.1
Coordinates 12242..12472 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC22_RS23465 (7379) 7379..7609 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
PIC22_RS23470 (7606) 7606..8022 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
PIC22_RS23475 (8067) 8067..11861 - 3795 WP_001144731.1 hypothetical protein -
PIC22_RS23480 (12242) 12242..12472 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
PIC22_RS23485 (12469) 12469..12885 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
PIC22_RS23490 (12960) 12960..14525 + 1566 WP_001128474.1 AAA family ATPase -
PIC22_RS23495 (14510) 14510..15532 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / tet(A) / qnrS1 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat 1..187674 187674
- flank IS/Tn - - 15786..16289 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T267884 WP_001034044.1 NZ_CP116089:12469-12885 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT267884 WP_001261286.1 NZ_CP116089:12242-12472 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References